Ple140-lacZ (Positive)

Ple140-lacZ Description:

This Ple140 promoter construct is derived from the promoter region of the Orphan Nuclear Receptor NR2E1 (NR2E1) gene. The Pleiades Promoter Project created a 4379 bp MiniPromoter derived from five upstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1612 with Ple140 driving lacZ. The Ple140-lacZ (pEMS1612) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated two ESC lines, mEMS4485, and mEMS4486. Chimeras were generated using mEMS4485.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple140 MiniPromoter Resources:

Ple140-lacZ
	plasmid pEMS1612 (in backbone pEMS1313)
	ESCs mEMS4485, mEMS4486
	mice generated using ESC mEMS4485
Resource Files
	Ple140 pEMS1612 Sequence	Jones lab
	Ple140 pEMS1612 MiniPromoter Design	Wasserman lab

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