Ple131-lacZ (Positive)*

Ple131-lacZ Description:

This Ple131 promoter construct is derived from the promoter region of the Antigen KI-67 (MKI67) gene. The Pleiades Promoter Project created a 3088 bp MiniPromoter derived from two upstream and one downstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1602 with Ple131 driving lacZ. The Ple131-lacZ (pEMS1602) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4655, mEMS4657, mEMS4658, and mEMS4659. Chimeras were generated using mEMS4655.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple131 MiniPromoter Resources:

Ple131-lacZ
	plasmid pEMS1602 (in backbone pEMS1313)
	ESCs mEMS4655, mEMS4657, mEMS4658, mEMS4659
	mice generated using ESC mEMS4655
Resource Files
	Ple131 pEMS1602 Sequence	Jones lab
	Ple131 pEMS1602 MiniPromoter Design	Wasserman lab
	Ple131 pEMS1602 Vector NTI File  (Requires VectorNTI Sofware)	Holt lab
	Ple131 pEMS1602 Vector Map	Holt lab