Ple129-lacZ (Positive)

Ple129-lacZ Description:

The Ple129 promoter construct is derived from the promoter region of the Antigen KI-67 (MKI67) gene. The Pleiades Promoter Project created a 3900 bp MiniPromoter derived from three upstream putative regulatory subregion along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1600 with Ple129 driving lacZ. The Ple129-lacZ (pEMS1600) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4342, mEMS4362, mEMS4370, and mEMS4345. Chimeras were generated using mEMS4342.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple129 MiniPromoter Resources:

Ple129-lacZ
	plasmid pEMS1600 (in backbone pEMS1313)
	ESCs mEMS4342, mEMS4362, mEMS4370, mEMS4345
	chimeras generated using ESC mEMS4342
Resource Files
	Ple129 pEMS1600 Sequence	Jones lab
	Ple129 pEMS1600 MiniPromoter Design	Wasserman lab
	Ple129 pEMS1600 Vector NTI File  (Requires VectorNTI Sofware)	Holt lab
	Ple129 pEMS1600 Vector Map	Holt lab