Ple32-lacZ (Positive)

Ple32-lacZ Description:

The Ple32 promoter construct is derived from the promoter region of the Claudin 5 (CLDN5) gene. The Pleiades Promoter Project created a 1670 bp MiniPromoter derived from a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1503 with Ple32 driving lacZ. The Ple32-lacZ (pEMS1503) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated three ESC lines, mEMS4730, mEMS4728, and mEMS4729. Chimeras were generated using mEMS4730.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple32 MiniPromoter Resources:

Ple32-lacZ
	plasmid pEMS1503 (in backbone pEMS1313)
	ESCs mEMS4730, mEMS4728, mEMS4729
	chimeras generated using ESC mEMS4730
Resource Files
	Ple32 pEMS1503 Sequence	Jones lab
	Ple32 pEMS1503 MiniPromoter Design	Wasserman lab
	Ple32 pEMS1503 Vector NTI File  (Requires VectorNTI Sofware)	Holt lab
	Ple32 pEMS1503 Vector Map	Holt lab

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