Ple30-lacZ (Negative)
|
|
Other constructs for this MiniPromoter: Ple30-EGFP (Negative) |
|
Other constructs for this Gene: NONE |
|
|
Ple30-lacZ Description:
The Ple30 promoter construct is derived from the promoter region of the Macrosialin precursor (CD68) gene. The Pleiades Promoter Project created a 836 bp MiniPromoter derived from an upstream candidate regulatory region and an intronic candidate regulatory region, along with a segment of the promoter and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites) to make plasmid pEMS1501 with Ple30 driving lacZ. The Ple30-lacZ (pEMS1501) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1202.02. Four ESC lines (mEMS3705, mEMS3706, mEMS3699, and mEMS3707) were PCR validated. Chimeras were generated using mEMS3705.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
|
|
|
Ple30-lacZ Expression Pattern:
No xGal reaction was detected in numerous brain regions and retina.
|
|
|
Ple30 MiniPromoter Resources:
|
|
|
Publications:| Reference | PubMed ID |
|---|
| O'Reilly D, Quinn CM, El-Shanawany T, Gordon S, Greaves DR. Multiple Ets factors and interferon regulatory factor-4 modulate CD68 expression in a cell type-specific manner. J Biol Chem. 2003 Jun 13;278(24):21909-19. Epub 2003 Apr 3. | 12676954 | | Lang R, Rutschman RL, Greaves DR, Murray PJ. Autocrine deactivation of macrophages in transgenic mice constitutively overexpressing IL-10 under control of the human CD68 promoter. J Immunol. 2002 Apr 1;168(7):3402-11. | 11907098 | | | | | | Laukkanen J, Lehtolainen P, Gough PJ, Greaves DR, Gordon S, Yla-Herttuala S. Adenovirus-mediated gene transfer of a secreted form of human macrophage scavenger receptor inhibits modified low-density lipoprotein degradation and foam-cell formation in macrophages. Circulation. 2000 Mar 14;101(10):1091-6. | 10715253 | | Greaves DR, Quinn CM, Seldin MF, Gordon S. Functional comparison of the murine macrosialin and human CD68 promoters in macrophage and nonmacrophage cell lines. Genomics. 1998 Nov 15;54(1):165-8. | 9806844 | | Sawka-Verhelle D, Escoubet-Lozach L, Fong AL, Hester KD, Herzig S, Lebrun P, Glass CK. PE-1/METS, an antiproliferative Ets repressor factor, is induced by CREB-1/CREM-1 during macrophage differentiation. J Biol Chem. 2004 Apr 23;279(17):17772-84. Epub 2004 Jan 30. | 14754893 | | O'Reilly D, Greaves DR. Cell-type-specific expression of the human CD68 gene is associated with changes in Pol II phosphorylation and short-range intrachromosomal gene looping. Genomics. 2007 Sep;90(3):407-15. Epub 2007 Jun 20. | 17583472 | | Su YR, Blakemore JL, Zhang Y, Linton MF, Fazio S. Lentiviral Transduction of ApoAI Into Hematopoietic Progenitor Cells and Macrophages. Applications to Cell Therapy of Atherosclerosis. Arterioscler Thromb Vasc Biol. 2008 May 22; [Epub ahead of print] | 18497309 | | Shi C, Sakuma M, Mooroka T, Liscoe A, Gao H, Croce KJ, Sharma A, Kaplan D, Greaves DR, Wang Y, Simon DI. Downregulation of the forkhead transcription factor Foxp1 is required for monocyte differentiation and macrophage function. Blood. 2008 Sep 17; [Epub ahead of print] | 18799727 | | Lin AA, Tripathi PK, Sholl A, Jordan MB, Hildeman DA. IFN-{gamma} signaling in macrophage-lineage cells regulates CNS inflammation and chemokine production. J Virol. 2009 Jun 10; [Epub ahead of print] | 19515766 | | Pillai MM, Hayes B, Torok-Storb B. Inducible transgenes under the control of the hCD68 promoter identifies mouse macrophages with a distribution that differs from the F4/80 and CSF-1R expressing populations. Exp Hematol. 2009 Sep 19; [Epub ahead of print] | 19772887 |
|
|
|
|
|
|
