The Pleiades Promoter Project

All MiniPromoters

Ple26-lacZ (Positive)

Other constructs for this MiniPromoter: NONE

Other constructs for this Gene: NONE


6.7 weeks, F0, 9% chimera, Female (entire series)	21.6 weeks, F0, 37% chimera, Male (entire series)	21.6 weeks, F0, 37% chimera, Male (entire series)	21.6 weeks, F0, 37% chimera, Male (entire series)	21.6 weeks, F0, 37% chimera, Male (entire series)

Ple26-lacZ Description:

The Ple26 promoter construct is derived from the promoter region of the Small Inducible Cytokine A27 precursor (CCL27) gene. The Pleiades Promoter Project created a 3532 bp MiniPromoter derived from one upstream and two downstream putative regulatory subregions along with a section of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 (LacZ reporter flanked by full frt sites (WT and F5 mutant)) to make plasmid pEMS1497 with Ple26 driving lacZ. The Ple26-lacZ (pEMS1497) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. The resulting PCR validated four ESC lines, mEMS4643, mEMS4644, mEMS4649, and mEMS4654. Chimeras were generated using mEMS4643.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple26-lacZ Expression Pattern:

Ple26 MiniPromoter Resources:

Ple26-lacZ
	plasmid pEMS1497 (in backbone pEMS1313)
	ESCs mEMS4643, mEMS4644, mEMS4649, mEMS4654
	chimeras generated using ESC mEMS4643
Resource Files
	Ple26 pEMS1497 Sequence	Jones lab
	Ple26 pEMS1497 MiniPromoter Design	Wasserman lab
	Ple26 pEMS1497 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple26 pEMS1497 Vector Map	Holt lab