The Pleiades Promoter Project

All MiniPromoters

CAG-lacZ (Positive)

Other Constructs for CAG: CAG-EGFP (pEMS1157; Positive), CAG-EGFP (pEMS1277; Positive)


7.4 weeks, N2, Male (entire series)	11.3 weeks, N2, Male (entire series)

CAG-lacZ Description:

The Pleiades Promoter Project obtained the CAG promoter (a composite of the Chicken beta-actin promoter and 5' UTR, along with a Human CMV enhancer) and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1313 to make plasmid pEMS1488 with CAG driving constitutive expression of lacZ. The CAG-lacZ (pEMS1488) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X Chromosome of mouse embryonic stem cells (ESCs) mEMS1202. One ESC line (mEMS3960) was PCR validated. Chimeras were generated using mEMS3960.
This is a positive control.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have also been generated.

CAG Promoter Resources:

CAG-lacZ
	plasmid pEMS1488 (in backbone pEMS1313)
	ESCs mEMS3960
	mice generated using ESC mEMS3960
Resource Files
	CAG Sequence	Jones lab
	CAG MiniPromoter Design	Wasserman lab

Publications:

Reference	PubMed ID
Miyazaki J, Takaki S, Araki K, Tashiro F, Tominaga A, Takatsu K, Yamamura K. Expression vector system based on the chicken beta-actin promoter directs efficient production of interleukin-5. Gene. 1989 Jul 15;79(2):269-77.	2551778
Niwa H, Yamamura K, Miyazaki J. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene. 1991 Dec 15;108(2):193-9.	1660837

CAG-lacZ (Positive)

CAG-lacZ Description:

CAG-lacZ Expression Pattern:

CAG Promoter Resources:

Publications: