The Pleiades Promoter Project

All MiniPromoters

Ple67-EGFP (Positive)

Other constructs for this MiniPromoter: NONE

Other constructs for this Gene: Ple66-EGFP (Negative)


8.1 weeks, N2, Male (entire series)	8.1 weeks, N2, Male (entire series)

Ple67-EGFP Description:

The Ple67 promoter construct is derived from the promoter region of the FEV (ETS Oncogene Family) (FEV) gene. The Pleiades Promoter Project created a 2242bp MiniPromoter derived from a subsection of the FEV promoter. This construct was introduced into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1401 with Ple67 driving EGFP. The Ple67-EGFP (pEMS1401) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1204. Four ESC lines (mEMS2784, mEMS2788, mEMS2789, mEMS2790 ) were PCR validated. Chimeras were generated using mEMS2784.

Note: a promoterless negative control (pEMS1306) has been generated, as well as a CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple67 MiniPromoter Resources:

Ple67-EGFP
	plasmid pEMS1401 (in backbone pEMS1306)
	ESCs mEMS2784, mEMS2788, mEMS2789, mEMS2790
	mice generated using ESC mEMS2784
	The mouse strain is available through the Jackson Laboratory: http://jaxmice.jax.org/strain/009349.html
Resource Files
	Ple67 pEMS1401 Sequence	Jones lab
	Ple67 pEMS1401 MiniPromoter Design	Wasserman lab
	Ple67 pEMS1401 Vector NTI File (Requires VectorNTI Sofware)	Holt lab
	Ple67 pEMS1401 Vector Map	Holt lab
	Ple67 pEMS1401 Genotyping Assay	Simpson lab

Publications:

Reference	PubMed ID
Scott MM, Krueger KC, Deneris ES. A differentially autoregulated Pet-1 enhancer region is a critical target of the transcriptional cascade that governs serotonin neuron development. J Neurosci. 2005 Mar 9;25(10):2628-36.	15758173
Scott MM, Wylie CJ, Lerch JK, Murphy R, Lobur K, Herlitze S, Jiang W, Conlon RA, Strowbridge BW, Deneris ES. A genetic approach to access serotonin neurons for in vivo and in vitro studies. Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16472-7. Epub 2005 Oct 26.	16251278
Pfaar H, von Holst A, Vogt Weisenhorn DM, Brodski C, Guimera J, Wurst W. mPet-1, a mouse ETS-domain transcription factor, is expressed in central serotonergic neurons. Dev Genes Evol. 2002 Feb;212(1):43-6. Epub 2002 Jan 31.	11875656
Maurer P, Rorive S, de Kerchove d'Exaerde A, Schiffmann SN, Salmon I, de Launoit Y. The Ets transcription factor Fev is specifically expressed in the human central serotonergic neurons. Neurosci Lett. 2004 Mar 11;357(3):215-8.	15003288
Iyo AH, Porter B, Deneris ES, Austin MC. Regional distribution and cellular localization of the ETS-domain transcription factor, FEV, mRNA in the human postmortem brain. Synapse. 2005 Sep 15;57(4):223-8.	15986391
Krueger KC, Deneris ES. Serotonergic Transcription of Human FEV Reveals Direct GATA Factor Interactions and Fate of Pet-1-Deficient Serotonin Neuron Precursors. J Neurosci. 2008 Nov 26;28(48):12748-58.	19036967

Ple67-EGFP (Positive)

Ple67-EGFP Description:

Ple67-EGFP Expression Pattern:

Ple67 MiniPromoter Resources:

Publications: