Ple162-EGFP (Negative)
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Other constructs for this MiniPromoter: Ple162-EGFP/cre (Positive) |
Other constructs for this Gene: Ple160-EGFP/cre (Negative), Ple161-EGFP/cre (Negative),
Ple160-lacZ (Positive), Ple160-EGFP (Negative), Ple161-EGFP (Negative) |
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Ple162-EGFP Description:
This Ple162 promoter construct is derived from the promoter region of the Pituitary homeobox 3 (PITX3) gene. The Pleiades Promoter Project created a 3636 bp MiniPromoter derived from an intronic candidate regulatory region and a segment of the PITX3 promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1358 with Ple162 driving EGFP. The Ple162-EGFP (pEMS1358) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1204.03A. Two ESC lines (mEMS1679 and mEMS1680) were PCR validated. Chimeras were generated using mEMS1679.
Note: a promoterless negative control (pEMS1306)
has been generated, as well as a CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
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Ple162-EGFP Expression Pattern:
No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions (brightfield).
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Ple162 MiniPromoter Resources:
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Publications:| Reference | PubMed ID |
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| Semina EV, Murray JC, Reiter R, Hrstka RF, Graw J. Deletion in the promoter region and altered expression of Pitx3 homeobox gene in aphakia mice. Hum Mol Genet. 2000 Jul 1;9(11):1575-85. | 10861284 | | Smidt MP, Smits SM, Bouwmeester H, Hamers FP, van der Linden AJ, Hellemons AJ, Graw J, Burbach JP. Early developmental failure of substantia nigra dopamine neurons in mice lacking the homeodomain gene Pitx3. Development. 2004 Mar;131(5):1145-55. | 14973278 | | Smidt MP, van Schaick HS, Lanctot C, Tremblay JJ, Cox JJ, van der Kleij AA, Wolterink G, Drouin J, Burbach JP. A homeodomain gene Ptx3 has highly restricted brain expression in mesencephalic dopaminergic neurons. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13305-10. | 9371841 | | Rieger DK, Reichenberger E, McLean W, Sidow A, Olsen BR. A double-deletion mutation in the Pitx3 gene causes arrested lens development in aphakia mice. Genomics. 2001 Feb 15;72(1):61-72. | 11247667 | | Maxwell SL, Ho HY, Kuehner E, Zhao S, Li M. Pitx3 regulates tyrosine hydroxylase expression in the substantia nigra and identifies a subgroup of mesencephalic dopaminergic progenitor neurons during mouse development. Dev Biol. 2005 Jun 15;282(2):467-79. | 15950611 | | Coulon V, L'Honore A, Ouimette JF, Dumontier E, van den Munckhof P, Drouin J. A muscle-specific promoter directs Pitx3 gene expression in skeletal muscle cells. J Biol Chem. 2007 Nov 9;282(45):33192-200. Epub 2007 Sep 11. | 17848564 | | Zhao S, Maxwell S, Jimenez-Beristain A, Vives J, Kuehner E, Zhao J, O'Brien C, de Felipe C, Semina E, Li M. Generation of embryonic stem cells and transgenic mice expressing green fluorescence protein in midbrain dopaminergic neurons. Eur J Neurosci. 2004 Mar;19(5):1133-40. | 15016072 | | Castillo-Carranza DL, Rodriguez-Rocha H, Montes-de-Oca-Luna R, Sepulveda-Saavedra J, Martinez HR, Lopez-Vidal Y, Saucedo-Cardenas O. Pitx3 promoter directs Cre-recombinase specifically in a human neuroblastoma cell line. Mol Cell Biochem. 2008 Feb;309(1-2):223-7. Epub 2007 Nov 30. | 18049867 | | Hedlund EM, Pruszak J, Lardaro T, Ludwig W, Vinuela A, Kim KS, Isacson O. Embryonic Stem (ES) Cell-derived Pitx3-eGFP Midbrain Dopamine Neurons Survive Enrichment by FACS and Function in an Animal Model of Parkinson's Disease. Stem Cells. 2008 Apr 3; [Epub ahead of print] | 18388307 | | 0 | | Qiu HY, Guo C, Cheng XW, Huang Y, Xiong ZQ, Ding YQ. Pitx3-CreER mice showing restricted Cre expression in developing ocular lens and skeletal muscles. Genesis. 2008 Jun;46(6):324-8. | 18543300 | | Doni A, Mantovani G, Porta C, Tuckermann J, Reichardt HM, Kleiman A, Sironi M, Rubino L, Pasqualini F, Nebuloni M, Signorini S, Peri G, Sica A, Beck-Peccoz P, Bottazzi B, Mantovani A. Cell-specific regulation of PTX3 by glucocorticoid hormones in hematopoietic and non-hematopoietic cells. J Biol Chem. 2008 Aug 14; [Epub ahead of print] | 18703503 | | Jacobs FM, Van der Linden AJ, Wang Y, von Oerthel L, Sul HS, Burbach JP, Smidt MP. Identification of Dlk1, Ptpru and Klhl1 as novel Nurr1 target genes in meso-diencephalic dopamine neurons. Development. 2009 Jun 10; [Epub ahead of print] | 19515692 |
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