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Ple153-EGFP (Negative)

Other constructs for this MiniPromoter: Ple153-lacZ (Positive)
Other constructs for this Gene: NONE
 
7.3 weeks, N2, Male
(entire series)
 
 

Ple153-EGFP Description:

The Ple153 promoter construct is derived from the promoter region of the Oxytocin-neurophysin 1 precursor (OXT) gene. The Pleiades Promoter Project constructed a 3051 bp MiniPromoter from two downstream putative regulatory elements and a subsection of the OXT promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1238 with Ple153 driving EGFP. The Ple153-EGFP (pEMS1238) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS1204. Four ESC lines (mEMS2406, mEMS2407, mEMS2408, and mEMS2410) were PCR validated, and chimeras were generated using mEMS2406.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

 

Ple153-EGFP Expression Pattern:

No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions (brightfield).

 

Ple153 MiniPromoter Resources:

Ple153-EGFP
    plasmid pEMS1238 (in backbone pEMS1306)
ESCs mEMS2406, mEMS2407, mEMS2408, mEMS2410
mice generated using ESC mEMS2406
Resource Files
 Ple153 pEMS1238 SequenceJones lab
 Ple153 pEMS1238 MiniPromoter DesignWasserman lab
 Ple153 pEMS1238 Vector NTI File  (Requires VectorNTI Sofware)Holt lab
 Ple153 pEMS1238 Vector MapHolt lab
 

Publications:

ReferencePubMed ID
Fields RL, House SB, Gainer H. Regulatory domains in the intergenic region of the oxytocin and vasopressin genes that control their hypothalamus-specific expression in vitro. J Neurosci. 2003 Aug 27;23(21):7801-9. 12944509
Ratty AK, Jeong SW, Nagle JW, Chin H, Gainer H, Murphy D, Venkatesh B. A systematic survey of the intergenic region between the murine oxytocin- and vasopressin-encoding genes. Gene. 1996 Sep 26;174(1):71-8. 8863731
Richard S, Zingg HH. Identification of a retinoic acid response element in the human oxytocin promoter. J Biol Chem. 1991 Nov 15;266(32):21428-33. 1657967
Young WS 3rd, Iacangelo A, Luo XZ, King C, Duncan K, Ginns EI. Transgenic expression of green fluorescent protein in mouse oxytocin neurones. J Neuroendocrinol. 1999 Dec;11(12):935-9. 10583728
Zhang BJ, Kusano K, Zerfas P, Iacangelo A, Young WS 3rd, Gainer H. Targeting of green fluorescent protein to secretory granules in oxytocin magnocellular neurons and its secretion from neurohypophysial nerve terminals in transgenic mice. Endocrinology. 2002 Mar;143(3):1036-46. 11861530
0
Transgenesis and neuroendocrine physiology: a transgenic rat model expressing growth hormone in vasopressin neurones. Comment in: J Physiol. 2003 Aug 15;551(Pt 1):3. J Physiol. 2003 Aug 15;551(Pt 1):323-36. Epub 2003 Jun 17. 12813157
Ghorbel MT, Coulson JM, Murphy D. Cross-talk between hypoxic and circadian pathways: cooperative roles for hypoxia-inducible factor 1alpha and CLOCK in transcriptional activation of the vasopressin gene. Mol Cell Neurosci. 2003 Mar;22(3):396-404. 12691740
Adan RA, Burbach JP. Regulation of vasopressin and oxytocin gene expression by estrogen and thyroid hormone. Prog Brain Res. 1992;92:127-36. 1302872
Lipkin SM, Nelson CA, Glass CK, Rosenfeld MG. A negative retinoic acid response element in the rat oxytocin promoter restricts transcriptional stimulation by heterologous transactivation domains. Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1209-13. 1311087
Adan RA, Cox JJ, van Kats JP, Burbach JP. Thyroid hormone regulates the oxytocin gene. J Biol Chem. 1992 Feb 25;267(6):3771-7. 1371278
Gainer H, Wray S. Oxytocin and vasopressin. From genes to peptides. Ann N Y Acad Sci. 1992 Jun 12;652:14-28. 1626825
Mohr E, Schmitz E. Functional characterization of estrogen and glucocorticoid responsive elements in the rat oxytocin gene. Brain Res Mol Brain Res. 1991 Mar;9(4):293-8. 1645432
Schmitz E, Mohr E, Richter D. Rat vasopressin and oxytocin genes are linked by a long interspersed repeated DNA element (LINE): sequence and transcriptional analysis of LINE. DNA Cell Biol. 1991 Mar;10(2):81-91. 1706187
Adan RA, Walther N, Cox JJ, Ivell R, Burbach JP. Comparison of the estrogen responsiveness of the rat and bovine oxytocin gene promoters. Biochem Biophys Res Commun. 1991 Feb 28;175(1):117-22. 1998497
Richard S, Zingg HH. The human oxytocin gene promoter is regulated by estrogens. J Biol Chem. 1990 Apr 15;265(11):6098-103. 2108152
Hara Y, Battey J, Gainer H. Structure of mouse vasopressin and oxytocin genes. Brain Res Mol Brain Res. 1990 Oct;8(4):319-24. 2176709
Beekman JM, Cooney AJ, Elliston JF, Tsai SY, Tsai MJ. A rapid one-step method to purify baculovirus-expressed human estrogen receptor to be used in the analysis of the oxytocin promoter. Gene. 1994 Sep 2;146(2):285-9. 8076833
Burbach JP, Lopes da Silva S, Cox JJ, Adan RA, Cooney AJ, Tsai MJ, Tsai SY. Repression of estrogen-dependent stimulation of the oxytocin gene by chicken ovalbumin upstream promoter transcription factor I. J Biol Chem. 1994 May 27;269(21):15046-53. 8195142
Adan RA, Cox JJ, Beischlag TV, Burbach JP. A composite hormone response element mediates the transactivation of the rat oxytocin gene by different classes of nuclear hormone receptors. Mol Endocrinol. 1993 Jan;7(1):47-57. 8383287
Lopes da Silva S, Adan RA, Burbach JP. Transcriptional regulation of the rat oxytocin promoter. Activation and suppression by members of the steroid/thyroid hormone receptor family. Ann N Y Acad Sci. 1993 Jun 11;684:227-9. 8391239
Burbach JP, Adan RA. The rat oxytocin gene. Physiological changes in expression in the hypothalamo-neurohypophysial system and responsiveness of promoter activity. Ann N Y Acad Sci. 1993 Jul 22;689:34-49. 8396870
Burbach JP, Adan RA, Cox JJ, da Silva SL. Transactivation of the rat oxytocin and vasopressin promoters by nuclear hormone receptors. Regul Pept. 1993 Apr 29;45(1-2):31-5. 8511362
Bale TL, Dorsa DM. Cloning, novel promoter sequence, and estrogen regulation of a rat oxytocin receptor gene. Endocrinology. 1997 Mar;138(3):1151-8. 9048622
Waller SJ, Ratty A, Burbach JP, Murphy D. Transgenic and transcriptional studies on neurosecretory cell gene expression. Cell Mol Neurobiol. 1998 Apr;18(2):149-71. 9535288
Chu K, Boutin JM, Breton C, Zingg HH. Nuclear orphan receptors COUP-TFII and Ear-2: presence in oxytocin-producing uterine cells and functional interaction with the oxytocin gene promoter. Mol Cell Endocrinol. 1998 Feb;137(2):145-54. 9605516
Gainer H. Cell-specific gene expression in oxytocin and vasopressin magnocellular neurons. Adv Exp Med Biol. 1998;449:15-27. 10026782
Chu K, Zingg HH. Activation of the mouse oxytocin promoter by the orphan receptor RORalpha. J Mol Endocrinol. 1999 Dec;23(3):337-46. 10601979
Sladek CD, Swenson KL, Kapoor R, Sidorowicz HE. The role of steroid hormones in the regulation of vasopressin and oxytocin release and mRNA expression in hypothalamo-neurohypophysial explants from the rat. Exp Physiol. 2000 Mar;85 Spec No:171S-177S. 10795920
Wood JR, Likhite VS, Loven MA, Nardulli AM. Allosteric modulation of estrogen receptor conformation by different estrogen response elements. Mol Endocrinol. 2001 Jul;15(7):1114-26. 11435612
Loven MA, Likhite VS, Choi I, Nardulli AM. Estrogen response elements alter coactivator recruitment through allosteric modulation of estrogen receptor beta conformation. J Biol Chem. 2001 Nov 30;276(48):45282-8. Epub 2001 Sep 26. 11574541
Stedronsky K, Telgmann R, Tillmann G, Walther N, Ivell R. The affinity and activity of the multiple hormone response element in the proximal promoter of the human oxytocin gene. J Neuroendocrinol. 2002 Jun;14(6):472-85. 12047722
Shughrue PJ, Dellovade TL, Merchenthaler I. Estrogen modulates oxytocin gene expression in regions of the rat supraoptic and paraventricular nuclei that contain estrogen receptor-beta. Prog Brain Res. 2002;139:15-29. 12436923
Murphy D, Wells S. In vivo gene transfer studies on the regulation and function of the vasopressin and oxytocin genes. J Neuroendocrinol. 2003 Feb;15(2):109-25. 12535153
Young WS 3rd, Gainer H. Transgenesis and the study of expression, cellular targeting and function of oxytocin, vasopressin and their receptors. Neuroendocrinology. 2003 Oct;78(4):185-203. 14583651
Gould BR, Zingg HH. Mapping oxytocin receptor gene expression in the mouse brain and mammary gland using an oxytocin receptor-LacZ reporter mouse. Neuroscience. 2003;122(1):155-67. 14596857
Koohi MK, Ivell R, Walther N. Transcriptional activation of the oxytocin promoter by oestrogens uses a novel non-classical mechanism of oestrogen receptor action. J Neuroendocrinol. 2005 Apr;17(4):197-207. 15842231
Ye X, Li Y, Huang Q, Yu Y, Yuan H, Wang P, Wan D, Gu J, Huo K, Li YY, Lu H. The novel human gene MIP functions as a co-activator of hMafF. Arch Biochem Biophys. 2006 May 15;449(1-2):87-93. Epub 2006 Mar 6. 16549056
Soloff MS, Izban MG, Cook DL Jr, Jeng YJ, Mifflin RC. Interleukin-1-induced NF-kappaB recruitment to the oxytocin receptor gene inhibits RNA polymerase II-promoter interactions in cultured human myometrial cells. Mol Hum Reprod. 2006 Oct;12(10):619-24. Epub 2006 Aug 3. 16888077
Wang CP, Lee YF, Chang C, Lee HJ. Transactivation of the proximal promoter of human oxytocin gene by TR4 orphan receptor. Biochem Biophys Res Commun. 2006 Dec 8;351(1):204-8. Epub 2006 Oct 12. 17054912
 
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