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Ple39-EGFP (Negative)

Other constructs for this MiniPromoter: NONE
Other constructs for this Gene: NONE
 
8 weeks, N2, Male
(entire series)
 
 

Ple39-EGFP Description:

The Ple39 promoter construct is derived from the promoter region of the Corticoliberin precursor (Corticotropin-releasing factor) (CRF) gene. The Pleiades Promoter Project created a 3929 bp MiniPromoter derived from an upstream candidate regulatory region and a segment of the promoter and 5' UTR of the gene and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1306 (EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1156 with Ple39 driving EGFP. The Ple39-EGFP (pEMS1156) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS21TG2A. Three ESC lines (mEMS2135, mEMS2161, and mEMS2167) were PCR validated. Chimeras were generated using mEMS2167.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

 

Ple39-EGFP Expression Pattern:

No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions (brightfield).

 

Ple39 MiniPromoter Resources:

Ple39-EGFP
    plasmid pEMS1156 (in backbone pEMS1306)
ESCs mEMS2136, mEMS2161, mEMS2167
mice generated using ESC mEMS2167
 

Publications:

ReferencePubMed ID
Keegan CE, Herman JP, Karolyi IJ, O'Shea KS, Camper SA, Seasholtz AF. Differential expression of corticotropin-releasing hormone in developing mouse embryos and adult brain. Endocrinology. 1994 Jun;134(6):2547-55. 8194481
Vamvakopoulos NC, Karl M, Mayol V, Gomez T, Stratakis CA, Margioris A, Chrousos GP. Structural analysis of the regulatory region of the human corticotropin releasing hormone gene. FEBS Lett. 1990 Jul 2;267(1):1-5. 2365075
Spengler D, Rupprecht R, Van LP, Holsboer F. Identification and characterization of a 3',5'-cyclic adenosine monophosphate-responsive element in the human corticotropin-releasing hormone gene promoter. Mol Endocrinol. 1992 Nov;6(11):1931-41. 1480179
Vamvakopoulos NC, Chrousos GP. Evidence of direct estrogenic regulation of human corticotropin-releasing hormone gene expression. Potential implications for the sexual dimophism of the stress response and immune/inflammatory reaction. J Clin Invest. 1993 Oct;92(4):1896-902. 8408641
Scatena CD, Adler S. Trans-acting factors dictate the species-specific placental expression of corticotropin-releasing factor genes in choriocarcinoma cell lines. Endocrinology. 1996 Jul;137(7):3000-8. 8770924
Guardiola-Diaz HM, Kolinske JS, Gates LH, Seasholtz AF. Negative glucorticoid regulation of cyclic adenosine 3', 5'-monophosphate-stimulated corticotropin-releasing hormone-reporter expression in AtT-20 cells. Mol Endocrinol. 1996 Mar;10(3):317-29. 8833660
Malkoski SP, Handanos CM, Dorin RI. Localization of a negative glucocorticoid response element of the human corticotropin releasing hormone gene. Mol Cell Endocrinol. 1997 Mar 28;127(2):189-99. 9099914
Scatena CD, Adler S. Characterization of a human-specific regulator of placental corticotropin-releasing hormone. Mol Endocrinol. 1998 Aug;12(8):1228-40. 9717848
Wolfl S, Martinez C, Majzoub JA. Inducible binding of cyclic adenosine 3',5'-monophosphate (cAMP)-responsive element binding protein (CREB) to a cAMP-responsive promoter in vivo. Mol Endocrinol. 1999 May;13(5):659-69. 10319317
Malkoski SP, Dorin RI. Composite glucocorticoid regulation at a functionally defined negative glucocorticoid response element of the human corticotropin-releasing hormone gene. Mol Endocrinol. 1999 Oct;13(10):1629-44. 10517666
Cheng YH, Nicholson RC, King B, Chan EC, Fitter JT, Smith R. Glucocorticoid stimulation of corticotropin-releasing hormone gene expression requires a cyclic adenosine 3',5'-monophosphate regulatory element in human primary placental cytotrophoblast cells. J Clin Endocrinol Metab. 2000 May;85(5):1937-45. 10843178
Cheng YH, Nicholson RC, King B, Chan EC, Fitter JT, Smith R. Corticotropin-releasing hormone gene expression in primary placental cells is modulated by cyclic adenosine 3',5'-monophosphate. J Clin Endocrinol Metab. 2000 Mar;85(3):1239-44. 10720069
King BR, Smith R, Nicholson RC. Novel glucocorticoid and cAMP interactions on the CRH gene promoter. Mol Cell Endocrinol. 2002 Aug 30;194(1-2):19-28. 12242024
Vamvakopoulos NC, Chrousos GP. Regulated activity of the distal promoter-like element of the human corticotropin-releasing hormone gene and secondary structural features of its corresponding transcripts. Mol Cell Endocrinol. 1993 Jul;94(1):73-8. 8397123
Vamvakopoulos NC, Chrousos GP. Structural organization of the 5' flanking region of the human corticotropin releasing hormone gene. DNA Seq. 1993;4(3):197-206. 8161822
King BR, Nicholson RC. Advances in understanding corticotrophin-releasing hormone gene expression. Front Biosci. 2007 Jan 1;12:581-90. 17127319
Guardiola-Diaz HM, Boswell C, Seasholtz AF. The cAMP-responsive element in the corticotropin-releasing hormone gene mediates transcriptional regulation by depolarization. J Biol Chem. 1994 May 20;269(20):14784-91. 8182084
Van LP, Spengler DH, Holsboer F. Glucocorticoid repression of 3',5'-cyclic-adenosine monophosphate-dependent human corticotropin-releasing-hormone gene promoter activity in a transfected mouse anterior pituitary cell line. Endocrinology. 1990 Sep;127(3):1412-8. 1696884
Seasholtz AF, Thompson RC, Douglass JO. Identification of a cyclic adenosine monophosphate-responsive element in the rat corticotropin-releasing hormone gene. Mol Endocrinol. 1988 Dec;2(12):1311-9. 2851101
Punn A, Levine MA, Grammatopoulos DK. Identification of signaling molecules mediating corticotropin-releasing hormone-R1alpha-mitogen-activated protein kinase (MAPK) interactions: the critical role of phosphatidylinositol 3-kinase in regulating ERK1/2 but not p38 MAPK activation. Mol Endocrinol. 2006 Dec;20(12):3179-95. Epub 2006 Sep 7. 16959871
Yamamori E, Iwasaki Y, Taguchi T, Nishiyama M, Yoshida M, Asai M, Oiso Y, Itoi K, Kambayashi M, Hashimoto K. Molecular mechanisms for corticotropin-releasing hormone gene repression by glucocorticoid in BE(2)C neuronal cell line. Mol Cell Endocrinol. 2007 Jan 29;264(1-2):142-8. Epub 2006 Dec 13. 17169483
Thompson RC, Seasholtz AF, Herbert E. Rat corticotropin-releasing hormone gene: sequence and tissue-specific expression. Mol Endocrinol. 1987 May;1(5):363-70. 3274895
King BR, Nicholson RC. Advances in understanding corticotrophin-releasing hormone gene expression. Front Biosci. 2007 Jan 1;12:581-90. 17127319
Ni X, Hou Y, King BR, Tang X, Read MA, Smith R, Nicholson RC. Estrogen receptor-mediated down-regulation of corticotropin-releasing hormone gene expression is dependent on a cyclic adenosine 3',5'-monophosphate regulatory element in human placental syncytiotrophoblast cells. J Clin Endocrinol Metab. 2004 May;89(5):2312-8. 15126558
Van LP. Phorbolester stimulates the activity of human corticotropin-releasing hormone gene promoter via 3',5'-cyclic adenosine monophosphate response element in transiently transfected chicken macrophages. Endocrinology. 1993 Jan;132(1):30-4. 8380380
Ni X, Hou Y, Yang R, Tang X, Smith R, Nicholson RC. Progesterone receptors A and B differentially modulate corticotropin-releasing hormone gene expression through a cAMP regulatory element. Cell Mol Life Sci. 2004 May;61(9):1114-22. 15112058
Adler GK, Smas CM, Majzoub JA. Expression and dexamethasone regulation of the human corticotropin-releasing hormone gene in a mouse anterior pituitary cell line. J Biol Chem. 1988 Apr 25;263(12):5846-52. 2965706
Foradori CD, Lund TD, Nagahara AH, Koenig JI, Handa RJ. Corticotropin-releasing hormone heterogeneous nuclear RNA (hnRNA) and immunoreactivity are induced in extrahypothalamic brain sites by kainic-acid-induced seizures and are modulated by estrogen. Brain Res. 2007 Aug 20;1164:44-54. Epub 2007 Jun 12. 17631870
Chen XN, Zhu H, Meng QY, Zhou JN. Estrogen receptor-alpha and -beta regulate the human corticotropin-releasing hormone gene through similar pathways. Brain Res. 2008 May 27; [Epub ahead of print] 18597742
Chen Y, Cheung YT, Kong LD, Ng TB, Qiao C, Mo SF, Xu HX, Kung HF. Transcriptional regulation of corticotrophin releasing factor gene by furocoumarins isolated from seeds of Psoralea corylifolia. Life Sci. 2008 May 23;82(21-22):1117-21. Epub 2008 Apr 1. 18445497
Liu Y, Kamitakahara A, Kim AJ, Aguilera G. Cyclic adenosine 3',5'-monophosphate responsive element binding protein phosphorylation is required but not sufficient for activation of corticotropin-releasing hormone transcription. Endocrinology. 2008 Jul;149(7):3512-20. Epub 2008 Mar 27. 18372325
Uh A, Nicholson RC, Gonzalez GV, Simmons CF, Gombart A, Smith R, Equils O. Lipopolysaccharide stimulation of trophoblasts induces corticotropin-releasing hormone expression through MyD88. Am J Obstet Gynecol. 2008 Sep;199(3):317.e1-6. 18771998
Blume A, Torner L, Liu Y, Subburaju S, Aguilera G, Neumann ID. Prolactin activates MAP kinase signaling and corticotropin releasing hormone (CRH) transcription in rat hypothalamic neurons. Endocrinology. 2008 Nov 20; [Epub ahead of print] 19022892
van der Laan S, de Kloet ER, Meijer OC. Timing is critical for effective glucocorticoid receptor mediated repression of the cAMP-induced CRH gene. PLoS ONE. 2009;4(1):e4327. Epub 2009 Jan 29. 19177170
Pereira JH, Kim SH. Structure of human Brn-5 transcription factor in complex with CRH gene promoter. J Struct Biol. 2009 May 15; [Epub ahead of print] 19450691
Uh A, Simmons CF, Bresee CT, Khoury N, Gombart AF, Nicholson RC, Kocak H, Equils O. MyD88 and TRIF mediate the cyclic adenosine monophosphate (cAMP) induced corticotropin releasing hormone (CRH) expression in JEG3 choriocarcinoma cell line. Reprod Biol Endocrinol. 2009 Jul 17;7(1):74 [Epub ahead of print] 19615077
Fick LJ, Fick GH, Belsham DD. Rhythmic clock and neuropeptide gene expression in hypothalamic mHypoE-44 neurons. Mol Cell Endocrinol. 2010 Mar 6; [Epub ahead of print] 20211689
 
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