Ple105-EGFP/cre (Negative)

Ple105-EGFP/cre Description:

This Ple105 promoter construct is derived from the promoter region of the huntingtin-associated protein (HAP1) gene. The Pleiades Promoter Project created a 2355 bp MiniPromoter derived from a putative intron-based regulatory element and a subsection of the promoter, and introduced them into the multiple cloning site (MCS) of Pleiades expression vector pEMS1302 (EGFP/cre reporter flanked by minimal frt sites) to make plasmid pEMS1085 with Ple105 driving EGFP/cre. The Ple105-EGFP/cre (pEMS1085) was introduced by homologous recombination as a single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS21. Four ESC lines (mEMS942, mEMS943, mEMS944, and mEMS945) were PCR validated. Chimeras were generated using mEMS942.

Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313) have been generated, as well as CAG positive controls (pEMS1157, pEMS1277 & pEMS1488).

Ple105-EGFP/cre Expression Pattern:

MiniPromoter Ple105 was assayed for LacZ and EGFP from pEMS1302 backbone. No xGal reaction was detected in numerous brain regions and retina. No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions and retina (brightfield).

Ple105 MiniPromoter Resources:

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