Ple99-cre/EGFP (Negative)
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Other constructs for this MiniPromoter: NONE |
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Other constructs for this Gene: NONE |
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Ple99-cre/EGFP Description:
This Ple99 promoter construct is derived from the promoter region of the gastrin releasing peptide (GRP) gene. The Pleiades Promoter Project constructed a 1418 bp MiniPromoter derived from a subsection of the promoter and introduced it into the multiple cloning site (MCS) of Pleiades expression vector pEMS1301 (cre/EGFP reporter flanked by minimal frt sites) to make plasmid pEMS1071 with Ple99 driving cre/EGFP. The Ple99-cre/EGFP (pEMS1071) was introduced by homologous recombination as single copy insertion into the Hprt1b-m3 locus in the X chromosome of mouse embryonic stem cells (ESCs) mEMS21. Four ESC lines (mEMS733, mEMS735, mEMS736, and mEMS738) were PCR validated. Chimeras were generated using mEMS733.
Note: Promoterless negative controls (pEMS1302, pEMS1306, pEMS1313)
have been generated, as well as CAG positive controls (pEMS1157,
pEMS1277 & pEMS1488).
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Ple99-cre/EGFP Expression Pattern:
MiniPromoter Ple99 was assayed for LacZ and EGFP from pEMS1301 backbone. No xGal reaction was detected in numerous brain regions and retina. No EGFP expression was detected above background in native fluorescence under epi or confocal microscopy. Anti GFP immunostaining (rabbit polyclonal, Invitrogen) did not detect specific GFP expression above background across numerous brain regions and retina (brightfield).
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Ple99 MiniPromoter Resources:
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Publications:| Reference | PubMed ID |
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| Inase N, Horita K, Tanaka M, Miyake S, Ichioka M, Yoshizawa Y. Use of gastrin-releasing peptide promoter for specific expression of thymidine kinase gene in small-cell lung carcinoma cells. Int J Cancer. 2000 Mar 1;85(5):716-9. | 10699954 | | Lebacq-Verheyden AM, Way J, Battey J. Structural characterization of a brain-specific promoter region directing transcription of the rat prepro-gastrin-releasing peptide gene. Brain Res Mol Brain Res. 1990 Apr;7(3):235-41. | 2159583 | | Godley JM, Brand SJ. Regulation of the gastrin promoter by epidermal growth factor and neuropeptides. Proc Natl Acad Sci U S A. 1989 May;86(9):3036-40. | 2566164 | | Markowitz S, Krystal G, Lebacq-Verheyden AM, Way J, Sausville EA, Battey J. Transcriptional activation and DNase I hypersensitive sites are associated with selective expression of the gastrin-releasing peptide gene. J Clin Invest. 1988 Sep;82(3):808-15. | 2843572 | | Lebacq-Verheyden AM, Krystal G, Sartor O, Way J, Battey JF. The rat prepro gastrin releasing peptide gene is transcribed from two initiation sites in the brain. Mol Endocrinol. 1988 Jun;2(6):556-63. | 2843761 | | Sausville EA, Lebacq-Verheyden AM, Spindel ER, Cuttitta F, Gazdar AF, Battey JF. Expression of the gastrin-releasing peptide gene in human small cell lung cancer. Evidence for alternative processing resulting in three distinct mRNAs. J Biol Chem. 1986 Feb 15;261(5):2451-7. | 3003116 | | Nagalla SR, Spindel ER. Functional analysis of the 5'-flanking region of the human gastrin-releasing peptide gene in small cell lung carcinoma cell lines. Cancer Res. 1994 Aug 15;54(16):4461-7. | 8044796 | | Spindel ER, Zilberberg MD, Chin WW. Analysis of the gene and multiple messenger ribonucleic acids (mRNAs) encoding human gastrin-releasing peptide: alternate RNA splicing occurs in neural and endocrine tissue. Mol Endocrinol. 1987 Mar;1(3):224-32. | 2840564 | | Wada E, Way J, Lebacq-Verheyden AM, Battey JF. Neuromedin B and gastrin-releasing peptide mRNAs are differentially distributed in the rat nervous system. J Neurosci. 1990 Sep;10(9):2917-30. | 2398368 | | Xiao D, Wang J, Hampton LL, Weber HC. The human gastrin-releasing peptide receptor gene structure, its tissue expression and promoter. Gene. 2001 Feb 7;264(1):95-103. | 11245983 | | Pedersen N, Pedersen MW, Lan MS, Breslin MB, Poulsen HS. The insulinoma-associated 1: a novel promoter for targeted cancer gene therapy for small-cell lung cancer. Cancer Gene Ther. 2006 Apr;13(4):375-84. | 16052225 | | Zhang X, Yang JJ, Kim YS, Kim KY, Ahn WS, Yang S. An 8-gene signature, including methylated and down-regulated glutathione peroxidase 3, of gastric cancer. Int J Oncol. 2010 Feb;36(2):405-14. | 20043075 |
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